Application of these assays to 231 Nonpareil × Lauranne F 1 progeny provided robust linkage maps for each parent. Here, we report on the application of genotyping by sequencing to discover and map single nucleotide polymorphisms in the almond cultivars “Nonpareil” and “Lauranne.” Allele-specific marker assays were developed for 309 tag pairs. Webb), application of a double pseudotestcross mapping approach to the F 1 progeny of a biparental cross leads to the construction of a linkage map for each parent. In outcrossing species such as almond ( Prunus dulcis Mill. In crop plant genetics, linkage maps provide the basis for the mapping of loci that affect important traits and for the selection of markers to be applied in crop improvement. The genotypic and phenotypic data used for QTL analysis are in Tables S12 and S13 in File S1. The best BLAST hits in the peach genome for SNP-bearing tags from Nonpareil and Lauranne are in Tables S9 and S10 in File S1, respectively. Results obtained from the application of KASP markers to almond clones and rootstocks are in Tables S8 and S11 in File S1, respectively. Linkage maps for Lauranne, Constantí, Tarraco, and Vairo are in Table S7 in File S1. Linkage maps for Nonpareil are in Table S6 in File S1. Primer sequences for KASP assays are in Table S5 in File S1. Contig sequences for Nonpareil have been deposited in the European Nucleotide Archive under accession number PRJEB23106. Barcode and primer sequences used for GBS are in Table S4 in File S1. Information on fragment size distributions from in silico digestion of the peach genome sequence is in Table S3 in File S1. Sequence data for 89 N × L progeny have been deposited in the National Center for Biotechnology Information Short Read Archive: study SRR5722967. Information on the parentage and origin of the almond clones and rootstocks used in this research is in Tables S1 and S2 in File S1.

Crack geneious trial